Uji Aktivitas Antioksidan dan Penetapan Kadar Flavonoid Total Pada Ekstrak Etanol Daun Bandotan (Ageratum conyziodes) dengan Metode DPPH

Mahral Effendi Sembiring; Lydia Br. Barus; Defacto Firmawati Zega; Nurbaiti Br. Singarimbun; Suharni Pintamas Sinaga; Elseria Saragih; Junita Manullang; Parsaoran Pardede; Sahat Sinaga; Helen Anjelina Simanjuntak; Herlina Simanjuntak

doi:10.58996/hmj.v9i2.254

Mahral Effendi Sembiring Program Studi Sarjana Farmasi, Fakultas Teknologi dan Kesehatan, Universitas Senior Medan, Indonesia
Lydia Br. Barus Program Studi Pendidikan Profesi Bidan, Fakultas Teknologi dan Kesehatan, Universitas Senior Medan, Indonesia
Defacto Firmawati Zega Program Studi Sarjana Kebidanan, Fakultas Teknologi dan Kesehatan, Universitas Senior Medan, Indonesia
Nurbaiti Br. Singarimbun Program Studi Sarjana Kebidanan, Fakultas Teknologi dan Kesehatan, Universitas Senior Medan, Indonesia
Suharni Pintamas Sinaga Program Studi Sarjana Kebidanan, Fakultas Teknologi dan Kesehatan, Universitas Senior Medan, Indonesia
Elseria Saragih Program Studi Sarjana Kebidanan, Fakultas Teknologi dan Kesehatan, Universitas Senior Medan, Indonesia
Junita Manullang Program Studi Sarjana Kebidanan, Fakultas Teknologi dan Kesehatan, Universitas Senior Medan, Indonesia
Parsaoran Pardede Program D-III Radiodiagnostik & Radioterapi, Fakultas Teknologi dan Kesehatan, Universitas Senior Medan, Indonesia
Sahat Sinaga Program D-III Radiodiagnostik & Radioterapi, Fakultas Teknologi dan Kesehatan, Universitas Senior Medan, Indonesia
Helen Anjelina Simanjuntak Program Studi Sarjana Farmasi, Fakultas Teknologi dan Kesehatan, Universitas Senior Medan, Indonesia
Herlina Simanjuntak Program Studi Sarjana Kebidanan, Fakultas Teknologi dan Kesehatan, Universitas Senior Medan, Indonesia

DOI: https://doi.org/10.58996/hmj.v9i2.254

Keywords: Ageratum conyzoides L, antioxidant, DPPH, total flavonoids, ethanol extract

Abstract

Daun bandotan, also known as Ageratum conyzoides L., contains flavonoids that may have anti-alami properties. The purpose of this study is to determine the total flavonoid content and anti-oxidant activity of the ekstrak etanol daun bandotan. With 96% ethanol as the pelarut, ekstraksi is carried out using the maserasi method. The DPPH (2,2-difenil-1-pikrilhidrazil) method is used to decrease antioksidan activity by decreasing absorption at 517 nm. To determine the total flavonoid content, spectrophotometry is used as a reference. The study's findings indicate that the antioksidan activity of ekstrak etanol daun bandotan is rather high, with an IC50 of 33,619 μg/mL in the Kadar category. The study's findings indicate that ekstrak etanol daun bandotan can function as an alami antioksidan, with an estimated 33,7938 mg of flavonoids per QE.